C. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. NanoRacks added it to their line of commercial research hardware on the International Space Station, providing researchers the ability to conduct microplate reader experiments in microgravity for the first time. 0 nm increments Bandwidth (EX, EM) 9 nm, 15 nm Optimized Precision ≤ 3. 5. 5. The corrected absorbance is within 5% of the. Bottom-reading is usually recommended for cell-based assay with adherent cells. In particular, these parameters should be entered as defined in the table below. Molecular Devices SpectraDrop ™ Micro-Volume Microplate allows users to read up to 64 samples per plate on SpectraMax ® Microplate Readers, with sample volumes as low as 2 µL. HTRF assay performance on the SpectraMax M5e reader is demonstrated here with two different HTRF assay kits, cAMP dynamic 2 and IP-One. Upgrades to the original platform include temperature control and the ability to configure every aspect of the researcher’s experiment fromM3-M5e_manual. Microplate Readers include the M3, M4,. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. Upgradeable modules are also available including western blot, cell imaging, and fast. 1. , contact Molecular. ® reader to the computer. Meso Scale Discovery 1200 Sector Imager 2400. 2 x 10-14. The analysis capabilities of the software provide automated quantitation in an easy-to-read, user-customizable report format. Conquer Scientific provides laboratories around the world high quality, low-cost pre-owned laboratory instruments. 3. Two cynomolgus monkeys (1 male and 1 female) weighing 2–4 kg and 3–5 years old were purchased from Suzhou. SpectraMax® iD5. Supplier: Molecular Devices. ZERO BIAS - scores, article reviews, protocol conditions and moreThe NanoRacks Plate Reader-2 is a reconfigured SpectraMax M5e, one of Molecular Devices’ most reliable, durable, and feature-rich microplate readers, and has been configured to operate comfortably in the microgravity environment of the space station. Toxicokinetics studies on cynomolgus monkey. The SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. 5 mP standard deviation at 1 nMOEM: Molecular DevicesMaterial: SpectraMax M5e Common Name: Microplate Spectrophotometer Item Weight: 36 Serial Number: MVE06577 SKU#: Inv-00352193. 1. 00 USD. 00 USD. Description 1. Transcreener® HTS is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes—many of which catalyze the covalent regulatory. SpectraMax Mini: 17. Conclusions. SpectraMax M5e Reader only Certified to Cisbio Bioassays HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. The BRD4 bromodomain 1 TR-FRET assay was performed as described in the assay kit manual. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seSpectraMax L: 36. SpectraMax M5e Multi-Mode Microplate Reader. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. If not, our scientific teamwill help you choose the instrument that best suits your needs and budget. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. GE AKTA™ Crossflow. The following document is. Both instruments demonstrated good performance for the assay. Homogeneous Time-Resolved Fluorescence (HTRF) . AquaMax® 4000. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Lift up the lid over the chamber to insert or remove a cuvette. SpectraMax M3, M4, M5, and M5 e. $. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. 2 x 10-14. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. reader (FI-FLRH) SpectraMax Paradigm. Ends from. Once the M5e Reader is operational, space-based researchers will be able to conduct analysis on-orbit. 5, Z’ = 0. After excluding the background values, the cross‐reactivity of the. SpectraMax M5e Multi-Mode Microplate Reader (Molecular Devices cat. combines Molecular Devices’ SpectraMax® M5e Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Jul 13, 2023 10am PT Date Format. Cytation™ 5 combines automated digital microscopy and conventional microplate detection in a configurable, upgradable platform. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. SpectraMax Paradigm Platform in conjunction with AlphaLISA provides a powerful tool to screen novel therapeutics in a pathologically relevant, endothelial cell-based model. Purchase of Additional 1 Year Service Plan includes: MRC Essential Service Plan, including parts, labor, and shipping/handling costs for emergency repairs. 8. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. 1. 3. A fluorescence microscope with high-throughput screening capabilities (Axio Observer. The SpectraMax iD5 and SpectraMax i3x readers use SoftMax® Pro Data Acquisition and Analysis Software. Moving a SpectraMax Multi-Mode Microplate Reader. On SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). SpectraMax M5e: 118. STAKMAX. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. LMax, SpectraMax ®, VersaMax™, VMax , EMax , UVMax, FlexStation®, and ThermoMax benchtop Microplate Readers. 2222 Fifth Street. The self-funded NanoRacksSpectraMax® Paradigm® Multi-Mode Detection Platform. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. Products : SoftMax Pro GxP Software, SoftMax Pro Software, SpectraMax M5/M5e reader. Today, Molecular Devices, a leading provider of instruments, software, and consumables for drug discovery and life sciences research, announced that NanoRacks, LLC has chosen the SpectraMax(R) M5e. M5 reader microplate m3 spectramax molecular devices llc m5e biocompare quote fluorescenceMolecular devices spectramax plus 384 user manual pdf download Spectramax® m2/m2e multimode microplate readers, with softmax® proMolecular devices spectramax m5 microplate reader labx contract svc warranty available manufacturer. View the Molecular Devices website. For more sample throughput, the system can Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The SpectraMax M5, M5e, and. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. Products : SpectraMax M3 reader, SoftMax Pro Software, SpectraMax M5/M5e reader, SpectraMax L reader, SpectraMax M4 reader Is the Gaussia Luciferase (GLuc) Assay from New England Biolabs compatible with my plate reader? The information for the GLuc Assay Kit (Cat no. IMAP assays are based on binding of. the performance of the Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader with an integrated 8-channel or 16-channel pipettor into one compact benchtop reader. We also buy, refurbish, and resell used lab equipment. Page 75. The SpectraMax M5e possesses five preferred modes for multiple detection benefits in one platform: UV-Visible Absorbance (Abs), Fluorescence Intensity (FI), Time-Resolved Fluorescence (TRF), Fluorescence. AquaMax® 2000. SpectraMax Mini: 17. FP assay do not perform well with protein:protein interactions. Dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. View full details Original price $5,513. reference drug program proton pump inhibitors (ppis) section 3 – diagnosis for requested medication gastroesophageal reflux disease (gerd), or reflux esophagitis, or duodenal. 0. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Enzyme titration and time course, cofactor and substrate Km,andIC50 for known inhibitors are demonstrated. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects andThe SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. The actual pathlength, d, of a solvent is found from the following equation: d cm. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 40266 Email: drugdiscoverytech@lifetech. 306 plate air cal fail,low light. Purchase of Additional 1 Year Service Plan includes: MRC Essential Service Plan, including parts, labor, and shipping/handling costs for emergency repairs. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax Paradigm reader. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. 4, Z’ = 0. 6 x 10-15. We would like to show you a description here but the site won’t allow us. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader $7,900. HTRF is compatible with a large range of available microplate readers. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. the SpectraMax M5e, one of Molecular Devices most reliable and feature-rich microplate readers, to operate comfortably in the zero-gravity environment of the space station. Commenting on the launch, Christopher Silva, Director of Life Sciences Marketing at Molecular Devices, stated, "The HTRF(R) assay platform has given researchers an alternative set of kinase and GPCR assays, in. 3. edu) to be added as a trained user in iLab. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. 1. 1, right panel), for rapid, real-time high throughput screening of mitochondrial (dys) function. KeySpan adapter for SoftMax Pro and SpectraMax readers. Page 8 FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Based on the SpectraMax M5e instrument platform, the FlexStation 3 instrument can address detection modalities including absorbance, fluorescence intensity, fluorescence polarization, time-resolved fluorescence, and luminescence. com A. SpectraMax Injector Cartridge with SmartInject™ Technology. The CellTiter-Glo® 2. NanoRacks reconfigured the SpectraMax M5e, one of Molecular Devices’ most reliable, durable, and feature-rich microplate readers, to operate comfortably in the zero-gravity environment of the space station. The SpectraDrop Microplate incorporates a specially designed adapter and a slide pair whose optical clarity allows measurements in absorbance and. 1. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. SpectraMax Mini: 17. 1. SpectraMax Mini: 17. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. 0001 0. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. ® reader to the computer. The optical systemsApplication note demonstrating that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. 005) Photometric Precision多功能酶标仪SpectraMax M5e. Note: In this user guide, SpectraMax ® refers to several SpectraMax . 6 x 10-15. G-protein coupled receptors signal through two major pathways, regulation of cAMP and increase in intracellular calcium levels mediated by IP3. Dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader Fluorescence Microplate Reader Comparison Chart Features We would like to show you a description here but the site won’t allow us. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. reader. For wavelengths in the UV range above 220 nm,. SpectraMax L: 36. For more sample throughput, the system canSetup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Rating: 5. 305 reference level saturation. HTRF cAMP calibration curve HTRF cAMP calibration curve measured on the SpectraMax i3 (red circles), SpectraMax. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seFigure 2: Expression of mCherry with (improved BBa_K1141000) and without (BBa_K1141000) the translation enhancing 5’ untranslated region was measured (Ex: 587 nm, Em: 610 nm) over a period of 6 hours after induction with 0. This user guide provides detailed instructions on how to operate, maintain, and troubleshoot the. D 1 1. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. 1, left panel) and individual wells (Fig. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. Features. The tunable SpectraMax M5e is a monochromator-based reader developed in collaboration with Cisbio international and is certified to run HTRF® assays. M3-M5e_manual. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. 5. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. This document is provided to customers who have purchased Molecular Devices, Inc. Sample Fluorescent Reader Setting for the ADP Hunter™ Plus (Cat. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 3. The donor and acceptor can be used to label a variety of biomolecules, for. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. The SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. reader (TUNE) SpectraMax M5e. M5e: W = 15. 5 Z’ factor 0. 1. 1(a). By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 1. SpectraMax M5e Multi-Mode Microplate Reader. In particular, these parameters should be entered as defined in the table below. e. MDC instruments including: SpectraMax®, VersaMax®, VMax®, EMax®, Gemini, ThermoMax®, and FlexStation®. NanoRacks LLC officials have chosen Molecular Devices’ SpectraMax M5e Multi-Mode Microplate Reader for use in NASA's International Space Station. Molecular Devices SpectraMax® series microplate readers. measured using a microplate reader (SpectraMax M5e, Molecular Devices, San Jose, CA, USA) with excitation and emission at 494 and 520 nm, respectively, and calibrated against a standard curve for FITC. Anne T. Note: In this user guide, SpectraMax ® refers to several SpectraMax . Skatron Skanwasher 300 Washer. 0 Cell Viability Assay exhibits the same high performance as the classic CellTiter-Glo® Assay, making it suitable for use from benchtop scale to high-throughput screening. book Page 1 Wednesday, July 7, 2010 2:09 PM FlexStation® 3 Benchtop Multi-Mode Microplate Reader User Guide 0112-0127 B June 2013 FlexStation_manual. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politicsSpectraMax® M5e Multi-Mode Microplate Reader (Molecular Devices) Assay setup and detection. Pick the best speed, sensitivity, and price for your needs. SpectraMax DuoLuc Reporter Assay (SMP 6. 0. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well NanoRacks, LLC is installing a new, reconfigured Molecular Devices’ SpectraMax M5e multi-mode microplate reader on the International Space Station to perform experiments in microgravity. Explore more about Optimized settings for Transcreener TR-FRET assay on SpectraMax iD5 and SpectraMax i3x readers. 72 MB. 40266 Email: [email protected]. In this. Optimal instrument settings are the same for all three readers. Bioz Stars score: 86/100, based on 1 PubMed citations. We would like to show you a description here but the site won’t allow us. Molecular Devices SpectraMax M5e User Manual (20 pages) Multi-Mode Microplate Readers. After washing, the chromogenic substrate was added, and the OD was read using a SpectraMax M5e microplate spectrophotometer (Molecular Devices) at 450 nm. These robust readers have been placed in labs from Antarctica to the. Supplier: Molecular Devices. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Delivers single mode reader performance in one multimode reader package. 92 0. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. WARNING! The instrument weighs approximately 36 pounds. 40266 Email: drugdiscoverytech@lifetech. 8 x 10-15. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. With the cAMP dynamic 2 kit, cAMP levels are detected using a Eu. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. M3,M4,M5,M5e,FlexStation 3 0200-6186 SpectraTest LM1 Luminescence ValidationPlate SpectraMaxiD3,iD5,i3x,i3,M3,M4,M5,M5e,SpectraMaxL, FlexStation 3 0200-2420Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. 6 x 10-15. Choose from a three- (M3), four- (M4), orSpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. • SpectraMax® M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate ReaderThe SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Description 1. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument. The optical systems use two scanning monochromators soThe SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. 标准功. Meso Scale Discovery 1250 Sector Imager 2400 $ 2,500. For more information about the SpectraMax® M5e Multi-Mode Microplate Reader or other products from Molecular Devices, please visit our website (or send an email to. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. 00 USD. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seSpectraMax L: 36. 1. Example: [Drawer]. book Page 1 Wednesday, July 7, 2010 2:09 PM. SpectraMax M 系列多功能微孔板读板机包括 M2/M2e、M3、M4、M5/M5e 等几款多功能酶标仪。. for Cisbio Bioassays HTRF® technology. Specifications Figure A-3 Top view of a SpectraMax reader ® Common Fluorescence and Luminescence Wavelengths Values in the following tables are based on the literature. Recommended Optics Wavelength (nm) Wavelength selection Excitation 332/9 Monochromator Emission 1 488/15. Instruments and Settings for Fluorescence Read-out (Other Fluorescent Assays) FLUOstar Omega, VANTAstar, CLARIOstar, PHERAstar, PolarStar Optima. SpectraMax® M5. With optimized reagents, option to operate the readers. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. 1. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator the assay on the SpectraMax Paradigm and M5e readers was evaluated by titration curves shown below. Molecular Devices SpectraMax M5e Microplate Reader. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Venue Address. SpectraMax M5. The optical systemsThe BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. Homogeneous Time-Resolved Fluorescence (HTRF) . 2 x 10-14. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. 5. SpectraMax M 系列多功能微孔板读板机（酶标仪）可测定紫外光 (UV) 和可见光 光吸收、荧光、发光、荧光偏振、时间分辨荧光 (TRF) 和均相时间分辨荧光 (HTRF) 。. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Description 1. HTRF测定在SpectraMax M5e读板机上的 表现通过使用两种不同的HTRF检测试剂 展示。G-蛋白偶联受体信号转导通过检测 两种主要通路，cAMP的调控和IP3介导的 胞内钙离子水平增加。利用cAMP dynamic 2试剂中Eu的穴状化合物标记的 单克隆抗-cAMP抗体和d2-标记的cAMP进 行检测。The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. SpectraMax M5e: 118. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. 3. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. 5. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuidethe SpectraMax M5e, one of Molecular Devices most reliable and feature-rich microplate readers, to operate comfortably in the zero-gravity environment of the space station. 8. Upgrades to the original platform include temperature control and the ability to configure every aspect of the researcher’s. 1. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. 28 nM to 0. SpectraMax M5e: 118. A neurosurgeon from Mercer Island and a noted mountaineer from Spokane, Washington, were killed on February 4, 1986, when. The large analog PMT works with monochromators and is used during fluorescence. Qualify the fluorescence performance of the SpectraMax . The optical systemsThe Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe SpectraMax M5e reader offers the additional benefit of being certified . 90 Read time (384 wells) 5:03 2:17 14:00 Table 3. The mirror set. At the American Heritage School, located in Plantation, Florida, students used a SpectraMax® M5e to conduct scientific research during their first Annual Summer Science Program. SpectraMax M5e: 118. Results summary for cAMP HiRange standard curve Figure 3. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precisionSpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. 0200-0729. Download PDF. 8. SpectraMax Quant dsDNA Assay Kits. Molecular Devices. Also refer to this Multimode Reader Comparison Chart. This version of Molecular Devices SpectraMax M3 Manual compatible with such list of devices, as: SpectraMax M3, SpectraMax M4, SpectraMax M5, SpectraMax M5eModel: SpectraMax™ M5e. Molecular Devices SpectraMax™ microplate readers, BioTek microplate washers, YMC (formerly LEWA) Process Technologies systems, ChromaCon Contichrom® instruments, and other niche laboratory equipment lines. Dual monochromators for assay flexibility. The mirror set. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seFor calcium content quantification, separate plates were used. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Find regional support. SpectraMax M5e: 118. Microplate Reader, For Lab. We specialize in GC, IC, GC/MS, HPLC, LC/MS, Spectrophotometer, and PCR Systems. SpectraMax Mini: 17. dual monochRomatoRs foR assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. Call 516-939-0888 to check current. 1. Introduction Inflammation, the body’s reaction to infection, irritation, or other injury, is accompanied by increased endothelial chemokine. M5e: W = 15. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 21 MB. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. 90-0083 series): Excitation 530 nm,. 7°C and the bottom to 36. Choose from a three- (M3), four- (M4), orMicroplate handling system for the spectramax paradigm multi-mode detection platform (110 pages). Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. D 1 1. Please see the exact video and photos below. Setup Guide. Add to cart. 00We would like to show you a description here but the site won’t allow us. book Page 1 Wednesday, July 7, 2010 2:09 PM. In the M2, M5 and Plus 384, the light. When the user applies a set temperature of 37°C, the top is heated to 37. 5. Read mode. In this application note we show how the SpectraMax ® i3, SpectraMax ® Paradigm ®, and SpectraMax ® M5e Multi-Mode Microplate Readers are used to perform robust, high-throughput HTRF ® assays with excellent Z’ factors and highly reproducible EC 50 values. book Page 1 Wednesday, July 7, 2010 2:09 PM SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. 1. Thermo Lab Systems Multiskan Ascent 354 $1,400. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. 0200-7027. When a feature or capability applies to only certain readers, this exception is noted. With optimized reagents, option to operate the. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. If you do not have an iLab account you must first make one: Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. The validation kit included individual tubes of diluted fluorophores: FAM, HEX, and FAM+HEX. Ex wavelength (nm/bandwidth) 485/20. Dual monochromators for assay flexibility. Path Check Pathlength Measurement Multimode Reader Comparison Chart. Your lab might even have one already. View the Molecular Devices website. About Conquer. This document is provided to customers who have purchased Molecular Devices, Inc. Molecular Devices SpectraMax M5e Microplate Reader $ 26,500. SpectraMax ABS Plus SpectraTest ABS2 v1. 5 mP standard deviation at 1. ZERO BIAS - scores, article reviews, protocol conditions and more-Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. Introduction. Upgrades to the original platform include temperature control and the ability to configure every aspect of the researcher’s experiment fromMolecular Devices SpectraMax™ M5e Microplate Reader w/ Warranty (Svc Contract Available) $34,000. Note: for the THUNDER assay, performance of a SpectraMax M5e reader will be equivalent to that of a SpectraMax M5 reader. This is a used Molecular Devices SpectraMax Gemini XS Microplate Fluorometer. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of. Z1. The plate was agitated briefly, incubated 15 minutes at room temperature in the dark and read on M5e. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. 91. Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. The NanoRacks Plate Reader-2 is a reconfigured SpectraMax M5e, one of Molecular Devices’ most reliable, durable, and feature-rich microplate readers, and has been configured to operate comfortably in the microgravity environment of the space station. Detection modes include: • Endpoint, kinetic, spectral and well-scanning • Comprehensive data analysis • Robotics compatibility • Fluorescence Intensity (FI) • Time-Resolved Fluorescence (TRF) The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology.